TILLING - a shortcut in functional genomics
نویسندگان
چکیده
منابع مشابه
TILLING: Harvesting functional genomics for crop improvement
Slade et al. describe the application of TILLING to the identification of an allelic series of variants in the granule-bound starch synthase I (GBSSI) gene in hexaploid and tetraploid wheat. GBSSI or Waxy plays a critical role in the synthesis of amylose, which, in addition to amylopectin, comprises the starch fraction of the seed. Reduction or loss of GBSSI function results in starch with a de...
متن کاملTILLING. Traditional mutagenesis meets functional genomics.
Most of the genes of an organism are known from sequence, but most of the phenotypes are obscure. Thus, reverse genetics has become an important goal for many biologists. However, reverse-genetic methodologies are not similarly applicable to all organisms. In the general strategy for reverse genetics that we call TILLING (for Targeting Induced Local Lesions in Genomes), traditional chemical mut...
متن کاملA TILLING Platform for Functional Genomics in Brachypodium distachyon
The new model plant for temperate grasses, Brachypodium distachyon offers great potential as a tool for functional genomics. We have established a sodium azide-induced mutant collection and a TILLING platform, called "BRACHYTIL", for the inbred line Bd21-3. The TILLING collection consists of DNA isolated from 5530 different families. Phenotypes were reported and organized in a phenotypic tree t...
متن کاملPerspectives on Translational Biology TILLING. Traditional Mutagenesis Meets Functional Genomics
Most of the genes of an organism are known from sequence, but most of the phenotypes are obscure. Thus, reverse genetics has become an important goal for many biologists. However, reverse-genetic methodologies are not similarly applicable to all organisms. In the general strategy for reverse genetics that we call TILLING (for Targeting Induced Local Lesions in Genomes), traditional chemical mut...
متن کاملTargeting induced local lesions IN genomes (TILLING) for plant functional genomics.
One of the most important breakthroughs in the history of genetics was the discovery that mutations can be induced (Muller, 1930; Stadler, 1932). The high frequency with which ionizing radiation and certain chemicals can cause genes to mutate made it possible to perform genetic studies that were not feasible when only spontaneous mutations were available. As a result, much of our understanding ...
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ژورنال
عنوان ژورنال: Journal of Applied Genetics
سال: 2011
ISSN: 1234-1983,2190-3883
DOI: 10.1007/s13353-011-0061-1